Evaluation of Anticancer, Antibacterial and Haemolytic Activities of Crude Mucus from Marine Catfish Tachysurus Dussumieri

Life Sciences-Zoology for Novel drug discovery

Abstract

Marine organisms are one of the diverse groups of animals that are highly specialized for their aquatic existence. The skin mucus share protective activities against environment that are rich in infectious agents. The current study aims to analyses the anticancer, antibacterial and haemolytic properties of crude epidermal mucus from marine catfish Tachysurus dussumieri.  Cell  viability  and  Cytotoxcity  was  assessed  by  MTT  method  on HT  29  (human  colon  carcinoma)  and  Vero (kidney epithelial cells of African Green Monkey) cell line. In Vero up to 1000 µg/ml of crude mucus shows cell viability of 80% and hence mucus concentration  for treatment of HT 29 cells was fixed between100  to 1000 µg/ml. The proliferation inhibition on HT 29 at a concentration of 400µg/ml mucus from T. dussumieri at 24 h. Cellular and nuclear morphological variations was observed in different concentration of mucus, followed by DNA fragmentation to confirm the induction of apoptosis. Moreover, mucus   sample   was   tested   against Gram   positive   and   negative bacteria, The maximum zone of inhibition was observed in S. aureus (15.61 ± 0.7 mm), followed by B. subtilis (8.32 ± 0.32 mm) and E. coli (8.14 ± 0.08 mm). Interestingly  mucus from T. dussumieri  showed haemolytic  activity on different  blood samples of goat, rat, sheep, buffalo, rabbit  ,  cow,  hen  and  human.  Mucus  showed  maximum  haemolytic  activity  against  goat,  rat,  sheep  and  buffalo  when compared to other RBCs. All these results showed that the epidermal mucus has more efficacy on therapeutic application in near future..