Biodegradation of sago effluent by white- rot fungus Phanerochaete chrysosporium

Life Sciences-Biotechnology for prospective medical science

Abstract

Phanerochaete chrysosporium was isolated by serial dilution from sago effluent taken from Attur Sago and Starch Treatment Plant Company Ltd, Tirupur, Tamilnadu, India.The NTG (N-Methyl N-Nitrosoguanidine) treated organisms with high concentration of glucose showed considerable increase in the sago effluent degradation. The mutated organisms were effective in degrading phenolic compounds and cyanide to a greater extent. In the absence of glucose, wild type isolates produced the least amount of enzyme. However, the concentration of glucose on wild type Phanerochaete chrysosporium could be increased in enzyme production. NTG was used to improve the efficiency of strain for better biodegradation. The efficiency of biodegraded sago effluent increases the rate of seed germination. The isolated Phanerochaete chrysosporium is therefore able to grow using diluted sago effluent as sole carbon source and also reduces the colour of the effluent, decreases chlorides, phenolic contents, COD, BOD and cyanides, thus providing an eco-friendly relation to the society. The effect of sago effluent to inhibit the growth of seeds was overcome by the treatment of the effluent with Phanerochaete chrysosporium. Sago effluent was chosen for the project, since it pollutes the ground water, after air pollution. Sago factories release large amount of effluent containing solid and liquid wastes. The heavy load of organic content and lower pH affects the soil. The aim of the present study was to analyse the degradation of effluent by Phanerochaete chrysosporium. A comparative analysis was done with biodegraded effluent and untreated effluents.