Preparation, Process Optimization and Cytotoxicity Evaluation of Lyophilized Etoposide Loaded Nanoparticles

Pharmaceutical Science-Pharmaceutics for effective drug dosage

Abstract

The purpose of this study was to develop freeze dried Polylactide-co-glycolide (PLGA) nanoparticles loaded with etoposide  intended  to be administered  intravenously  with improved  therapeutic  efficacy  of the drug and thereby  reduced dose related toxicity associated with it. The etoposide loaded nanoparticles were prepared using modified spontaneous emulsification solvent diffusion method (SESD).The in Vitro release study of etoposide loaded Polylactide-co-glycolide  (PLGA)–Nanoparticles  NP was carried  by dialysis  bag diffusion  technique.The  incorporation  efficiency  was found  to be higher  in modified  SESD  technique  than  emulsification  solvent  evaporation  method.  The  release  behavior  of etoposide  exhibited  a biphasic pattern characterized by an initial burst release followed by a slow and continuous release. The nanoparticles were characterized by particle size, zeta potential, polydispersity index, DSC and FTIR. The long term stability was achieved by lyophilisation  technique.  The cell line study using XTT assay on LNCaP  prostate  tumor  cell lines revealed  that etoposide loaded nanoparticles showed greater cancer cell inhibition compared to plain nanoparticles and marketed conventional formulations.