Evaluation of Antioxidant Property in Secondary Metabolites Isolated from Sargassum wightii Collected From Kanyakumari Coastal Region, Tamilnadu.

Life Sciences-Biotechnology

Abstract

The  demands  for  increasing  anticancer  drugs  produced  by  natural  sources  with  potential  antioxidant  and antimicrobial properties had led the path to mass production in pharmaceutical fields. This current investigation is implied to extract  the potential  crude  secondary  metabolites  extract  from  brown  algae  Sargassum  wightii  Greville  ex J.Agardh  with maximum antimicrobial and antioxidant properties. The brown seaweed was collected from Kanyakumari, Tamil Nadu,  India and taken for extraction process using various polar and non-polar solvents. The solvents which extracted maximum crude metabolites  were  taken  to determine  the preliminary  antimicrobial  analysis  using  clinical  pathogens  on  which  the  crude extracts  explored  the highest  zone of  inhibition  at  least  concentration. Further,  the five  different  crude  extracts  were subjected  to  antioxidant  assessment  and  the  extract  in  different  solvents  manifested  maximum  activity  at  minimum concentration. The results of antimicrobial and antioxidant analysis evinced that the ethyl acetate and methanolic extract exhibited maximum activity when compared to other crude extracts. The extract which explored the highest activity was taken for qualitative and quantitative phytochemical determination and both the extract showed the presence of most of the phytochemicals. Further, the investigation is carried out to purify and determine the anticancer property in cancer cell lines.