Sub-Acute Toxicity Study of a Type-I Pyrethroid Permethrin on Testis of Mus Musculus

Pharmaceutical Science-Pharmacology for Drug Discovery

Abstract

Pesticide usage has witnessed  a rise in the current years to overcome  the glitches of food scarcity because of incessant increase in population. Regardless of its extensive use in agriculture and related fields, these should be properly and thoroughly investigated because of unexplored danger which they bring along.  The present investigation deals about the effects of one such pesticide, Permethrin, belonging to the class of synthetic pyrethroids,  having broad range application in the field of agriculture, as an insect repellent, in lice shampoos, in the treatment of scabies and numerous other commercial uses. Although few reports have documented its adverse effect on the vital organs in different experimental models, there still persist lacunae in literature  about  its effect  on the male reproductive  tissue. Hence,  the present  study was aimed to evaluate  the effects of Permethrin on the male reproductive gonad of Swiss albino mice. Permethrin (130 mg/kg body weight) was administered orally for a period of 21 days after which the biochemical parameters in testis were assayed. The results showed that Permethrin administration lowered the gravimetric indices (body weight p<0.002, organ weight p<0.001) and decreased the levels of protein (p<0.001) and cholesterol (p<0.001), which is an important precursor in the formation of androgens.  Essential enzymes like 3β and 17β hydroxysteroid  dehydrogenase  which  are  pivotal  in  the  steroidogenesis  pathway  were  also  found  to be depleted (p<0.001 and p<0.002 respectively). These findings were further confirmed by the histopathological analysis and indicated that Permethrin had negative effects on the male reproductive system and can be one of the contributing factors leading to infertility.