Development of Rapid Detection and Estimation of Unexploited Cannabinoids from source Cannabis sativa and Cannabis indica by RP-HPLC

Pharmaceutical Science-Pharmaceutical Analysis for Better Drug Detection

Abstract

Siwan (Bihar) being a better surface to give loamy soil advances the development of a huge number of plants. Some basic hallucinogenic plants viz.Cannabis sativa L., and Cannabis indica (Lam.), were picked for the investigation of their dynamic constituents Tetrahydrocannabivarin, Cannabinol and Cannabichromene. Cannabis has recaptured a lot of consideration because of refreshed enactment approving a wide range of employments and can be characterized based on the substance of Tetrahydrocannabivarin (THCV), a psychotropic substance for which there are lawful impediments in numerous nations. For this reason, precise subjective and quantitative assurance is fundamental. The connection among Tetrahydrocannabinol (THC) and cannabidiol (CBD) is likewise critical as the last substance is invested with numerous particular and non-psychoactive legitimacies. Hence, it turns out to be progressively significant and earnest to use quick, simple,  approved  and  blended  systems  for  assurance  of  cannabinoids.  This  research  work  targeted  to  develop  quick standard operating protocol for characterizing cannabinoids from the hallucination causing plants of locale of Hathwa. The methodology portrayed in this permits fast assurance of 3-cannabinoids from the inflorescences of Cannabis sativa L. just as Cannabis indica (Lam.) by the extraction with certain solvents (Acetonitrile: Methanol ; 70:30). Detachment and ensuing recognition  are  by  RP-HPLC.  Evaluation  is  performed  by  an  outside  standard  technique  through  the  development  of adjustment blends utilizing unadulterated standard chromatogrape substance confirmation of Tetrahydro cannabivarin, Cannabinol and Cannabichromene were performed using reference mixtures. THCV was found to be the least amount. The limit of detection was 0.62 µg/ml, 0.32 µg/ml and 0.34 µg/ml for THCV, CAN and CBN respectively.