Hematological and Biochemical Investigations of Green Synthesized Reduced Graphene Oxide Carbon Nanoparticles on Acetaminophen Induced Male Albino Rats.

Life Sciences-Physiology for Better Medical Therapy

Abstract

The  aim  of  this  study  is  to  evaluate  the  protective  activity  of  green-synthesized   Reduced  graphene  oxide  carbon nanoparticles   (RGO-CNP)   from   Asparagus   racemosus   (shatamuli)   by   hematological   and   biochemical   assessments   on acetaminophen  induced uremic rats. 36 animals were divided into six groups including a control group, acetaminophen  induced uremic  group  and nanoparticle  treatment  groups.  Nanoparticle  treated  four groups  were intraperitonealyco-administered  RGO- CNP at doses of 0.5, 1.0, 2.0 and 4.0 mg/kg bw and acetaminophen  at 500 mg/kg ofbw for 14 days respectively.  Hematological analysis revealed that there was significant decrease (p<0.05) in Red Blood Cells (RBCs), Hemoglobin (Hb), with acetaminophen induced Group II.  Co-administration  of green synthesized  RGO and acetaminophen  on nanoparticle  treating groups showed the effective  protectivity  and  significant  (p<0.05)  recovery  in  the  hematological  alterations.  Other  hematological  parameters including- WBC, Platelet, Hematocrit, MCH and MCHC remained within the reference range and no significant alterations were observed.  There  was a significant  increase  in plasma  ALT, AST, serum urea and creatinine  levels  (p< 0.05)  in uremic  group (Group II) compare to control (Group I).The alterations were further resettled to the higher dose of RGO treated groups (Group V and VI). The other tested biochemical  parameters  ALP, total bilirubin, direct bilirubin, Serum triglyceride  and cholesterol  levels were not significantly  altered when compared  with control Group I. In conclusion,  it is established  that RGO possess protective response  against  acetaminophen  induced  toxicity  as  dose  responsive  manners,  which  is  supported  by  haematological  and biochemical  observations.  This  promising  effect  suggests  the  potential  applicability  of RGO  as therapeutics  in drug  delivery systems to reduce the toxicity.