EFFECT OF BACILLUS SUBTILIS AND LACTOBACILLUS RHAMNOSUS INCORPORATED PROBIOTIC DIET ON GROWTH PATTERN AND ENZYMES IN PENAEUS VANNAMEI

Life Science-Microbiology

Abstract

The effect of Bacillus subtilis and L.rhamnosus incorporated diet was investigated on growth pattern and antioxidant enzymes in Penaeus vannamei for a period of 3 weeks. 25- day old shrimps were stocked in three circular tanks. One of the tank fed with Lactobacillus rhamnosus incorporated diet and other tank was fed with Bacillus subtilis incorporated diet. The other tank was fed with commercial diet without probiotic which served as control group. After 3 weeks of treatment SGR, FCR, weight gain%, microbiological analysis and antioxidant enzyme concentration was determined. Statistical analysis was performed using analysis of variance (ANOVA) and Duncan’s new multiple range tests at P < 0.05 using spss 13 version. B.subtilis treated groups showed significant increase in WG % of (125 + 33 ) followed by L.rhamnosus treated groups of (93 + 0) followed by control group (42.8 +20).similar trend was observed with SGR. B.subtilis treated groups showed significant increase (1.25 ± 0.04) followed by L.rhamnosus showed (1.11±0.02) followed by control group (0.98±0.2). B.subtilis treated groups showed decreased FCR (1.90± 0.14) followed by L.rhamnosus treated groups showed (2.11± 0.52) and control group (2.74± 0.7). Total count of B.subtilis showed an increase of 7% compared to L.rhamnosus which was 4% after 3 weeks of feeding trial. The SOD activity of the haemolymph in shrimps fed with B.subtilis incorporated diet showed enhanced values of 2.4 auto-oxidation/min/mg of protein compared to L.rhamnosus 1.9 autooxidation/min/mg of protein that of control 1 auto- oxidation/min/mg of protein . The catalase activity of haemolymph in control was 1.09µ moles H2O2 decomposed -/min/mg of protein. The shrimp fed with B.subtilis incorporated diet showed the maximum catalase activity of 2.3µ moles H2O2 decomposed /min/mg of protein were as shrimps fed with L.rhamnosus incorporated diet showed catlase activity of 1.453µ moles H2O2 decomposed /min/mg of protein