STUDY OF ISOLATION OF LACCASE PRODUCING FUNGI AND ITS APPLICATION IN PLANT GROWTH PROMOTION
DOI:
https://doi.org/10.22376/ijlpr.v16i1.2031Keywords:
eco-friendly, laccase, lignin, lignocellulosic agricultural wastes, lvidopora benetosta, sustainableAbstract
The rapid accumulation of lignocellulosic agricultural wastes poses a serious environmental challenge due to its persistence during biodegradation and the release of pollutants during improper disposal. Lignin, a major structural component of agro-waste, is particularly resistant to conventional physical, chemical and biological methods of waste degradation. This study aimed to isolate and characterize an efficient laccase-producing fungal strain and evaluate its potential application in lignocellulosic waste bioconversion. Among the 65 isolates screened from 6 samples, only 4 isolates demonstrated laccase activity and Lividopora benetosta(NCBI accession No. OR262175) was identified as the most potential strain. Optimum production of laccase (1.23 U/mL) by L. benetostawas observed in potato dextrose broth (pH 7) containing 2% glucose,on incubation at room temperature under shaking conditions and absence of inducer (guaiacol).The practical applicability of the crude enzyme was assessed based on cocopeat treatment. Effective lignin degradation was observed on laccase application, and demonstratedfaster seed germination (within 1–2 days), increased root development, and reduced wilting compared to untreated controls in both monocot (Triticum aestivum) and dicot (Vigna radiata) plants. The T. aestivum seedlings showed approximately 25% larger shoot length, while V. radiata plants over 50% improvements in both shoot and root growth. These results demonstrate the potential of L. benetostaderived laccase as an eco-friendly and sustainable tool for agrowaste management and soil improvement.
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