Immunohistochemical Evaluation of Cytokeratin 17 Expression in Oral Squamous Cell Carcinoma
Life Sciences- Pathology
DOI:
https://doi.org/10.22376/ijlpr.2023.13.6.L411-L420Keywords:
Oral Squamous Cell Carcinoma, Cytokeratin 17, Immunohistochemistry, Broders Grading, Clinicopathologicparameters.Abstract
Oral squamous cell carcinoma (OSCC), which makes up 80–90% of all malignant neoplasms of the mouth, is the mostcommon type of oral cancer. Immunohistochemistry plays a crucial role in diagnostic pathology by aiding in identifying andclassifying tumors IHC is utilized to study the distribution and localization of specific proteins. Identifying diagnostic markers forOSCC is essential for early diagnosis and patient-specific treatment. Cytokeratins (CK) are a family of intermediate filamentspredominantly expressed in epithelial cells. The study aimed to evaluate immunohistochemical expression cytokeratin 17 in 50cases of microscopically confirmed OSCC. The present study was observational, with retrospective and prospective sampling.The study was carried out for two years, from 2020 to 2022. The present study was designed to determine CK 17 expressionby IHC and to correlate CK 17 expression with Broders grading of OSCC and other clinical pathologic parameters.Immunostaining for CK 17 was performed. The statistical analysis was done by chi-square test. In this study, there was asignificant correlation between CK 17 expression and Broders grading, and CK 17, when correlated with other clinic pathologicparameters, showed no significant correlation. It was concluded that Cytokeratin17 expression could correlate with theBroders' Grading, thus contributing to early detection and more accurate determination of tumor aggressiveness to determineappropriate therapy.
References
Markopoulos AK. Current aspects on oral squamous cell carcinoma. Open Dent J. 2012;6:126-30. doi: 10.2174/1874210601206010126, PMID 22930665.
Mehrotra R, Yadav S. Oral squamous cell carcinoma: etiology, pathogenesis and prognostic value of genomic alterations. Indian J Cancer. 2006;43(2):60-6. doi: 10.4103/0019-509x.25886. PMID 16790942.
Badvi JA, et al. Recent techniques for diagnosis of oral squamous cell carcinoma. EC Microbiol. 2017;5(5):165-8.
Bhargava A, Saigal S, Chalishazer M. Histopathological grading systems in oral squamous cell carcinoma: a review. JIOH. 2010;2(4).
Bonhin RG, de Carvalho GM, Guimarães AC, Chone CT, Crespo AN, Altemani AM et al. Histologic correlation of expression of Ki-67 in squamous cell carcinoma of the glottis according to the degree of cell differentiation. Braz J Otorhinolaryngol. 2014 Jul;80(4):290-5. doi: 10.1016/j.bjorl.2014.05.016, PMID 25183178.
Nambiar S, Hargannavar V, Augustine D. Immunohistochemistry: A brief Review. J Dent Oro-Facial Res. 2016;12(02).
Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M. Applications of immunohistochemistry. J Pharm Bioallied Sci. 2012;4;Suppl 2:S307-9. doi: 10.4103/0975-7406.100281. PMID 23066277.
Kitamura R, Toyoshima T, Tanaka H, Kawano S, Kiyosue T, Matsubara R, et al. Association of cytokeratin 17 expression with differentiation in oral squamous cell carcinoma. J Cancer Res ClinOncol. 2012 Aug;138(8):1299-310. doi: 10.1007/s00432-012-1202-6, PMID 22466643.
Jagannathan N. Cytokeratin: a review on current concepts. Int J Orofac Biol. 2018.
Pernick N, 2020. stains ck17. pathology outlines.com.
Koizumi A, Fifita SF, Kuyama K. Determination of immunohistochemical markers for oral squamous cell carcinoma Invasions. Indian j oral- med sci,. IJOMS. 2008;7(2):98-106. doi: 10.5466/ijoms.7.98.
Bancroft JD, Gamble M. Theory and practice of histological techniques. Elsevier Health Sciences; 2008.
Noguchi S, Sato K, Yamamoto G, Tonogi M, Tanaka Y, Tachikawa T, et al. Expression of cytokeratin 13 and 17 in tongue squamous cell carcinoma and epithelial dysplasia. Asian J Oral Maxillofac Surg. 2011;23(2):53-8. doi: 10.1016/j.ajoms.2010.12.005.
Moll R, Divo M, langbein L. The human keratins: biology and pathology. Histochem Cell Biol. 2008;129(6):705-33. doi: 10.1007/s00418-008-0435-6, PMID 18461349.
Wei KJ, Zhang L, Yang X, Zhong LP, Zhou XJ, Pan HY, et al. Overexpression of cytokeratin 17 protein in oral squamous cell carcinoma in vitro and in vivo. Oral Dis. 2009;15(1):111-7. doi: 10.1111/j.1601-0825.2008.01501.x, PMID 18992022.
Ikeda K, Tate G, Suzuki T, Mitsuya T. Coordinate expression of cytokeratin 8 and cytokeratin 17 immunohistochemical staining in cervical intraepithelial neoplasia and cervical squamous cell carcinoma: an immunohistochemical analysis and review of the literature. GynecolOncol. 2008;108(3):598-602. doi: 10.1016/j.ygyno.2007.11.042, PMID 18191996.
Cohen-Kerem R, Madah W, Sabo E, Rahat MA, Greenberg E, Elmalah I. Cytokeratin-17 as a potential marker for squamous cell carcinoma of the larynx. Ann OtolRhinolLaryngol. 2004;113(10):821-7. doi: 10.1177/000348940411301008, PMID 15535145.
Luo A, Kong J, Hu G, Liew CC, Xiong M, Wang X, et al. Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray. Oncogene. 2004;23(6):1291-9. doi: 10.1038/sj.onc.1207218, PMID 14647409.
Wetzels RH, Schaafsma HE, Leigh IM, Lane EB, Troyanovsky SM, Wagenaar SS, et al. Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18. Histopathology. 1992;20(4):295-303. doi: 10.1111/j.1365-2559.1992.tb00986.x, PMID 1374358.
Sanguansin S, Kosanwat T, Juengsomjit R, Poomsawat S. Diagnostic value of cytokeratin 17 during oral carcinogenesis: an immunohistochemical study. Int J Dent. 2021 Nov 22;2021:4089549. doi: 10.1155/2021/4089549, PMID 34853592.
Xu ES, Yang MH, Liu CY, Liu KW, Yang TT, Chou TY, et al. Decreasing cytokeratin 17 expression in head and neck cancer predicts nodal metastasis and poor prognosis: the first evidence. ClinOtolaryngol. 2018;43(4):1010-8. doi: 10.1111/coa.13092, PMID 29495101.
Coelho BA, Peterle GT, Santos M, Agostini LP, Maia LL, Stur E, et al. Keratins 17 and 19 expression as prognostic markers in oral squamous cell carcinoma. Genet Mol Res. 2015;14(4):15123-32. doi: 10.4238/2015.November.24.21, PMID 26634475.
Poschmann G, Sitek B, Sipos B, Ulrich A, Wiese S, Stephan C, et al. Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium. Mol Cell Proteomics. 2009;8(5):1105-16. doi: 10.1074/mcp.M800422-MCP200.
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Copyright (c) 2023 Dr. Ankita Gyanchandani, Dr. Samarth Shukla, Dr. RavindraKadu
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