The Correlation Between Serum Magnesium & Zinc with Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) In Patients with Metabolic Syndrome

Life Science-Physiology

Authors

  • Khaleel Ahmed Manik Research scholar, VMRF-Deemed to be University, Salem-636308, Tamil Nadu, India.
  • Sheela Joice P P Associate Professor, Dept of Physiology, Vinayaka Mission's Kirupananda Variyar Medical College & Hospital, VMRF-Deemed to be University, Salem-636308, Tamil Nadu, India.
  • Jithesh T K Associate Professor, Dept of Biochemistry, MES Medical College, Perinthalamanna, Kerala, India.
  • Imran A Jagadal Associate Professor, Dept of Physiology, KAHER’s JGMM Medical College, Hubballi, Karnataka. India.
  • Sudhindra Prathap. A Tutor, Dept of Pharmacology, SDM College of Medical Sciences and Hospital, Dharwad. Karnataka, India
  • Basheer M P Professor, Dept of Physiology, Al Azhar medical college and super specialty hospital

DOI:

https://doi.org/10.22376/ijlpr.2023.13.6.L227-L234

Keywords:

Metabolic Syndrome, Vascular Inflammation, Oxidative Stress, Lp-PLA2, Cardiovascular Disease, Micronutrients.

Abstract

Metabolic syndrome (MS) increases cardiovascular disease and death risk. Many studies have found a link between vascular inflammation and metabolic disorders. Discovering unique and specific blood-based indicators for vascular inflammation, particularly in metabolic syndrome related to obesity, such as (lipoprotein-associated phospholipase A2) and Lp-PLA2, could provide valuable assistance in identifying individuals at an elevated risk for cardiovascular incidents. Lp-PLA2 has been implicated in metabolic dysregulation, playing a crucial role in the onset of microvascular dysfunction and the exacerbation of oxidative stress. Lp-PLA2 is essential in the pathogenesis of atherosclerosis and may be used as a biomarker to predict future cardiovascular events. The study comprised 200 participants categorized into two groups: individuals diagnosed with MS (Metabolic Syndrome) (Test, n = 100) and those without MS (controls, n = 100). The serum activity levels of hs-CRP and Lp-PLA2 were measured and subsequently analysed for correlation with micronutrients (magnesium (Mg) and zinc (Zn)) and lipoprotein markers (Ox LDL, Apo-A1, and Apo-B). The study showed a significant correlation between Lp-PLA2 and the Mg level of patients with MS, whereas Hs-CRP did not exhibit a significant correlation. The test population did not exhibit a noteworthy elevation in oxidized LDL level, despite the presence of inflammatory changes as indicated by the level of Lp-PLA2. A significant correlation was observed between the Zn level in patients with MS and Lp-PLA2, whereas Ox LDL did not exhibit a significant correlation. The current study revealed a significant link between Mg and Zn and CVD risk in the Kerala population. The study found elevated levels of LpPLA2, an emerging biomarker for cardiovascular risk, in people with MS

References

Costa FF, Rosário WR, Ribeiro Farias AC, de Souza RG, Duarte Gondim RS, Barroso WA. Metabolic syndrome and COVID-19: an update on the associated comorbidities and proposed therapies. Diabetes Metab Syndr. 2020 Sep 1;14(5):809-14. doi 10.1016/j.dsx.2020.06.016, PMID 32540733.

Halim M, Halim A. The effects of inflammation, aging, and oxidative stress on the pathogenesis of diabetes mellitus (type 2 diabetes). Diabetes Metab Syndr Clin Res Rev. 2019 Mar 1;13(2):1165-72. doi: 10.1016/j.dsx.2019.01.040.

De Stefano A, Mannucci L, Tamburi F, Cardillo C, Schinzari F, Rovella V et al. Lp-PLA2, a new biomarker of vascular disorders in metabolic diseases. Int J Immunopathol Pharmacol. 2019;33:2058738419827154. doi: 10.1177/2058738419827154, PMID 30706739.

Pavithran N, Kumar H, Menon AS, Pillai GK, Sundaram KR, Ojo O. South Indian cuisine with low glycemic index ingredients reduces cardiovascular risk factors in subjects with type 2 diabetes. Int J Environ Res Public Health. 2020 Sep;17(17):6232. doi: 10.3390/ijerph17176232, PMID 32867226.

Kumric M, Borovac JA, Martinovic D, Ticinovic Kurir T, Bozic J. Circulating biomarkers reflecting coronary artery plaques destabilization mechanisms: are we looking for the impossible? Biomolecules. 2021 Jun;11(6):881. doi: 10.3390/biom11060881, PMID 34198543.

Pantazi D, Tellis C, Tselepis AD. Si: PAF. Oxidized Phospholipids Lipoprotein‐Assoc Phospholipase A2 (Lp‐PLA2) in atherosclerotic cardiovascular disease: An update. BioFactors. 2022 Oct 3.

Amer ASA, Al-Sheikh EHI, Fouda MHM, El-Hefnawi SB. Serum level of lipoprotein-associated phospholipase A2 and coronary artery disease. J Adv Med Med Res. 2022 Dec 27;34(24):151-9. doi: 10.9734/jammr/2022/v34i244914.

Okpara ES, Adedara IA, Guo X, Klos ML, Farombi EO, Han S. Molecular mechanisms associated with the chemoprotective role of protocatechuic acid and its potential benefits in the amelioration of doxorubicin-induced cardiotoxicity: a review. Toxicol Rep. 2022 Sep 7;9:1713-24. doi: 10.1016/j.toxrep.2022.09.001.

Genovesi S, Boriani G, Covic A, Vernooij RWM, Combe C, Burlacu A et al. Sudden cardiac death in dialysis patients: different causes and management strategies. Nephrol Dial Transplant. 2021 Mar;36(3):396-405. doi: 10.1093/ndt/gfz182, PMID 31538192.

Sharma P, Reddy PK, Kumar B. Trace element zinc, a nature’s gift to fight unprecedented global pandemic COVID-19. Biol Trace Elem Res. 2021 Sep;199(9):3213-21. doi: 10.1007/s12011-020-02462-8, PMID 33170448.

Pisoschi AM, Pop A, Iordache F, Stanca L, Predoi G, Serban AI. Oxidative stress mitigation by antioxidants – An overview of their chemistry and influences on health status. Eur J Med Chem. 2021 Jan 1;209:112891. doi: 10.1016/j.ejmech.2020.112891, PMID 33032084.

MacKenzie S, Bergdahl A. Zinc homeostasis in diabetes mellitus and vascular complications. Biomedicines. 2022 Jan 9;10(1):139. doi: 10.3390/biomedicines10010139, PMID 35052818.

Gadoa ZA, Moustafa AH, El Rayes SM, Arisha AA, Mansour MF. Zinc oxide nanoparticles and synthesized pyrazolopyrimidine alleviate diabetic effects in rats induced by Type II diabetes. ACS Omega. 2022 Oct 6;7(41):36865-72. doi: 10.1021/acsomega.2c05638, PMID 36278044.

Okafor OA. Studies on the role of zinc in maintaining antioxidant status in the heart [doctoral dissertation].

Marcovina SM, Albers JJ. Standardization of the Immunochemical Determination of Apolipoproteins A-I and B: a Report on the International Federation of Clinical Chemistry Meeting on Standardization of Apolipoprotein A-I and B Measurements (Basis for Future Consensus), Vienna, Austria, April 18-19, 1989. Clin Chem. 1989;35(9):2009-15. doi: 10.1093/clinchem/35.9.2009.

Vashist SK, Venkatesh AG, Marion Schneider EM, Beaudoin C, Luppa PB, Luong JH. Bioanalytical advances in assays for C-reactive protein. Biotechnol Adv. 2016;34(3):272-90. doi: 10.1016/j.biotechadv.2015.12.010, PMID 26717866.

Lp-PLA(2) Studies Collaboration, Thompson A, Gao P, Orfei L, Watson S, Di Angelantonio E et al. Lipoprotein-associated phospholipase A(2) and risk of coronary disease, stroke, and mortality: collaborative analysis of 32 prospective studies. Lancet. 2010;375(9725):1536-44. doi: 10.1016/S0140-6736(10)60319-4, PMID 20435228.

Friedman BJ, Freeland-Graves JH, Bales CW, Behmardi F, Shorey-Kutschke RL, Willis RA, et al. A comparison of standard methods for measuring water-soluble, ionizable zinc in human serum. Clin Chim Acta. 1987;163(2):201-10.

Lindner A, Zierz S, Kolbitsch C, Hebel S, Reiners C. New method for the measurement of magnesium using photometry and the dry chemistry module of the Ektachem 700 Analyzer. Clin Chem. 1990;36(4):712-6.

Matos J, Cardoso C, Bandarra NM, Afonso C. Microalgae as healthy ingredients for functional food: a review. Food Funct. 2017;8(8):2672-85. doi: 10.1039/c7fo00409e, PMID 28681866.

Picó C, Palou M, Pomar CA, Rodríguez AM, Palou A. Leptin as a key regulator of the adipose organ. Rev Endocr Metab Disord. 2022 Feb;23(1):13-30. doi: 10.1007/s11154-021-09687-5, PMID 34523036.

Luo Z, Xu W, Zhang Y, Di L, Shan J. A review of saponin intervention in metabolic syndrome suggests further study on intestinal microbiota. Pharmacol Res. 2020 Oct 1;160:105088. doi: 10.1016/j.phrs.2020.105088, PMID 32683035.

Pitzer Mutchler A, Huynh L, Patel R, Lam T, Bain D, Jamison S et al. The role of dietary magnesium deficiency in inflammatory hypertension. Front Physiol. 2023 May 24;14:1167904. doi 10.3389/Phys.2023.1167904, PMID 37293263.

Ambroselli D, Masciulli F, Romano E, Catanzaro G, Besharat ZM, Massari MC et al. New advances in metabolic syndrome, from prevention to treatment: the role of diet and food. Nutrients. 2023 Jan 26;15(3):640. doi: 10.3390/nu15030640, PMID 36771347.

Zhao X, An X, Yang C, Sun W, Ji H, Lian F. The crucial role and mechanism of insulin resistance in metabolic disease. Front Endocrinol. 2023;14:1149239. doi: 10.3389/fendo.2023.1149239, PMID 37056675.

Alexander J, Tinkov A, Strand TA, Alehagen U, Skalny A, Aaseth J. Early nutritional interventions with zinc, selenium and vitamin D for raising anti-viral resistance against progressive COVID-19. Nutrients. 2020 Aug 7;12(8):2358. doi: 10.3390/nu12082358, PMID 32784601.

Hou C, Zhang W, Li J, Du L, Lv O, Zhao S, et al. Beneficial effects of pomegranate on lipid metabolism in metabolic disorders. Mol Nutr Food Res. 2019 Aug;63(16):e1800773. doi: 10.1002/mnfr.201800773, PMID 30677224.

Bhatti JS, Sehrawat A, Mishra J, Sidhu IS, Navik U, Khullar N, et al. Oxidative stress in the pathophysiology of type 2 diabetes and related complications: current therapeutics strategies and future perspectives. Free Radic Biol Med. 2022 Apr 7;184:114-34. doi: 10.1016/j.freeradbiomed.2022.03.019, PMID 35398495.

Fathi M, Alavinejad P, Haidari Z, Amani R. The effects of zinc supplementation on metabolic profile and oxidative stress in overweight/obese patients with non-alcoholic fatty liver disease: A randomized, double-blind, placebo-controlled trial. J Trace Elem Med Biol. 2020 Dec 1;62:126635. doi: 10.1016/j.jtemb.2020.126635, PMID 32932174.

Schumann T, König J, Henke C, Willmes DM, Bornstein SR, Jordan J et al. Solute carrier transporters as potential targets for the treatment of metabolic disease. Pharmacol Rev. 2020 Jan 1;72(1):343-79. doi: 10.1124/pr.118.015735, PMID 31882442.

Anju T, Rai NKSR, Uthirchamkavu I, Sreedharan S, Ndhlala AR, Singh P et al. Analysis of nutritional and antioxidant potential of three traditional leafy vegetables for food security and human wellbeing. S Afr J Bot. 2022 Mar 1;145:99-110. doi: 10.1016/j.sajb.2021.11.042.

Gustafson D, Ngai M, Wu R, Hou H, Schoffel AC, Erice C et al. Cardiovascular signatures of COVID-19 predict mortality and identify barrier-stabilizing therapies. EBiomedicine. 2022 Apr 1;78:103982. doi: 10.1016/j.ebiom.2022.103982, PMID 35405523.

Kiyama R. Nutritional implications of ginger: chemistry, biological activities and signaling pathways. J Nutr Biochem. 2020 Dec 1;86:108486. doi: 10.1016/j.jnutbio.2020.108486, PMID 32827666.

Ke PH, Chen JY, Chen YH, Yeh WC, Li WC. Age- and sex-specific association between lipoprotein-related phospholipase A2 and cardiometabolic risk factors. Int J Mol Sci. 2023 Mar 30;24(7):6458. doi: 10.3390/ijms24076458, PMID 37047431.

Frąk W, Wojtasińska A, Lisińska W, Młynarska E, Franczyk B, Rysz J. Pathophysiology of Cardiovascular Diseases: New Insights into Molecular Mechanisms of Atherosclerosis, arterial hypertension, and coronary artery disease. Biomedicines. 2022 Aug 10;10(8):1938. doi: 10.3390/biomedicines10081938, PMID 36009488.

Published

2023-11-01

How to Cite

Manik, K. A., Joice P P , S., T K, J., A Jagadal, I., Prathap. A, S., & M P, B. (2023). The Correlation Between Serum Magnesium & Zinc with Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) In Patients with Metabolic Syndrome: Life Science-Physiology. International Journal of Life Science and Pharma Research, 13(6), L227-L234. https://doi.org/10.22376/ijlpr.2023.13.6.L227-L234

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Research Articles