Formulation and Evaluation of Herbal Cream for the Management of Androgenetic Alopecia
Pharmaceutical Science-Pharmacognosy
DOI:
https://doi.org/10.22376/ijlpr.2023.13.5.P78-P89Keywords:
Alopecia, 5α-reductase, HPTLC, herbal cream, Hibiscus rosa sinensis, Solanum nigrum, Eclipta alba.Abstract
The current study aimed to develop a herbal hair cream formulation including plant extracts traditionally used for hair growth, and further research was conducted to understand the hair growth capability better using the 5 alpha reductase inhibitory model. 5 alpha reductases, an enzyme confined to the nuclear membrane, converts testosterone into dihydrotestosterone. The 5 alpha-reductase and its metabolite dihydrotestosterone influence various human disorders, including male pattern baldness, benign prostatic hyperplasia (BPH), and prostate cancer. Finasteride and alfatradiol are examples of synthetic medications; however, because of their negative impacts, researchers are currently focused on creating safer 5α-reductase inhibitors from plants. The hair cream formulation contained 0.5% extracts of Hibiscus rosa sinensis flower, Eclipta alba whole plant, and Solanum nigrum plant berries in a ratio of 1:1:1. Plant extracts used in the formulation were characterized by chemical biomarkers. The cream formulation was developed, and the quality control parameters were examined. Furthermore, the produced formulation's 5α-reductase inhibitory activity was tested using finasteride as a positive control. H. rosa sinensis, E. alba, and S. nigrum extracts, which comprise 0.35% oleanolic acid, 4.65% β-sitosterol, and 31.18% linoleic acid, were used in the formulation. HPTLC data revealed that each 10 gm of cream formulation contains 16.68 ± 0.21 mg of linoleic acid, 3.61 ± 0.18 mg of β-sitosterol and 0.380 ± 0.11 mg oleanolic acid. In-vitro results showed a good 5α-reductase inhibitory potential. The IC50 value of the developed formulation was 85.254 ± 0.888 μg/ml, whereas finasteride (positive control) was 223.039 ± 1.367 ng/ml. Thus, the herbal hair cream composition benefits alopecia therapy by inhibiting the 5α- reductase enzyme. In addition, the formulation might be a potential option for further research into its antiandrogenic properties.
References
Sperling LC. Hair and systemic disease. DermatolClin. 2001;19(4):711-26. doi: 10.1016/S0733-8635(05)70310-7, PMID 11705356.
Kumar N, Chaiyasut C. Hair growth promoting activity of Carthamustinctorius florets extract-loaded nanostructured lipid carriers. Int J Pharm Pharm Sci. 2015;7(2):252-7.
Levy MA, Brandt M, Sheedy KM, Holt DA, Heaslip JI, Trill JJ, et al. Cloning, expression and functional characterization of type 1 and type 2 steroid 5α-reductase from Cynomolgus monkey: comparisons with human and rat isoenzymes. J Steroid BiochemMol Biol. 1995;52(4):307-19. doi: 10.1016/0960-0760(94)00183-M, PMID 7734398.
Nutbrown M, Hull SP, Cunliffe WJ, Randall VA. Abnormalities in the ultrastructure of melanocytes and the outer root sheath of clinically normal hair follicles from alopecia areata scalps. J Invest Dermatol. 1995;104(5);Suppl:12S-3S. doi10.1038/jid.1995.34. PMID 7738372.
Guarna A, Poletti A, Catrambone F, Danza G, Marrucci A, Serio M et al. Synthesis of a chemiluminescent probe useful for the purification of steroid 5α-reductase. Bioorg Med Chem Lett. 1996;6(16):1997-2002. doi: 10.1016/0960-894X(96)00360-5.
Patel S, Sharma V, Chauhan NS, Thakur M, Dixit VK. Hair growth: focus on an herbal therapeutic agent. Curr Drug Discov Technol. 2015;12(1):21-42. doi: 10.2174/1570163812666150610115055, PMID 26058803.
Duke JA. CRC Handbook of medicinal spices. CRC Press; 2002.
Sarma H, Sarma A. Solanumnigrum L., a nutraceutical enriched herb or invasive weed? International Conference on Environment and BioScience. Vol. 21; 2011. p. 105-9.
Saleem TM, Chetty C, Ramkanth S, Alagusundaram M, Gnanaprakash K, Rajan VT et al. Solanumnigrumlinn. A review. Pharmacogn Rev. 2009;3(6):342-5.
Caldecott T. Ayurveda: the divine science of life. Elsevier Health Sciences; 2006.
Roy RK, Thakur M, Dixit VK. Hair growth promoting activity of Eclipta alba in male albino rats. Arch Dermatol Res. 2008;300(7):357-64. doi: 10.1007/s00403-008-0860-3, PMID 18478241.
Upadhyay S, Upadhyay P. Hibiscus rosa-sinens is a pharmacological review. Int J Res Pharm. 2011;2(4):1449-50.
Upadhyay S, Upadhyay P, Vinode R, Dixit VK. Effect of an ethanolic fraction of Hibiscus rosasinensis l. leaves in androgenic alopecia. Egyptian. Dermatol Online J. 2013;9(5):1-7.
Adhirajan N, Ravi Kumar TR, Shanmugasundaram N, Babu M. In vivo and in vitro evaluation of hair growth potential of Hibiscus rosa-sinensis Linn. J Ethnopharmacol. 2003;88(2-3):235-9. doi: 10.1016/S0378-8741(03)00231-9, PMID 12963149.
Liu B, Chen X, Yi H, Han L, Ji B, Chen H, et al. β-catenin is involved in the oleanolic acid-dependent promotion of proliferation in human hair matrix cells in an in vitro organ culture model. Fitoterapia. 2017;121:136-40. doi: 10.1016/j.fitote.2017.07.007, PMID 28723343.
Ali M, Alam P, Singh V, Jameel M, Sultana S. Phytochemical investigations of the leaves and flowers of Hibiscus rosa-sinensis L. Indian Drugs. 2017;54(10):30-7. doi10.53879/id.54.10.11189.
Bhagure LB, Une HD. Identifying and quantifying oleanolic acid from Cycleapeltata by high-performance thin layer chromatography (HPTLC) method. Int J Pharm Sci Res. 2019;10(11):4899-06. doi: 10.13040/IJPSR.0975-8232.10(11).4899-06.
A B, A C, D D, A S, S C, N S et al.. Standardization of Eclipta Alba by HPTLC, HPLC, and AAS. Pharm Anal Acta;08(4). doi10.4172/2153-2435.1000541.
Chakraborty A, Bhattacharjee A, Dasgupta P. Simple Method for Standardization and Quantification of Linoleic Acid in Solanumnigrum Berries by HPTLC. J Chromatogr Sep Tech;07(6). doi10.4172/2157-7064.1000342.
Sahu AN, Jha SB, Dubey SD. Formulation & evaluation of curcuminoid-based herbal face cream. Indo-Glob Res J Pharm. 2011;1(1):77-84.
Dhase AS, Khadbadi SS, Saboo SS. Formulation and evaluation of vanishing herbal cream of crude drugs. Am J Ethnomed. 2014;1:313-8.
Bhowmik D, Gopinath H, Kumar BP, Kumar KS. Medicinal uses of Punicagranatum and its health benefits. J PharmacognPhytochem. 2013;1(5):28-35.
Nahata A, Dixit VK. Evaluation of the 5α‐reductase inhibitory activity of certain herbs useful as antiandrogens. Andrologia. 2014;46(6):592-601. doi: 10.1111/and.12115, PMID 23710567.
Chakraborty A, Bhattacharjee A. Herbal Hair Gel Formulation having 5α-Reductase Inhibitory Activity and its Standardization by HPTLC. J Anal Bioanal Tech;07(6). doi: 10.4172/2155-9872.1000341.
Niederprüm HJ, Schweikert HU, Zänker KS. Testosterone 5α-reductase inhibition by free fatty acids from Sabalserrulata fruits. Phytomedicine. 1994;1(2):127-33. doi: 10.1016/S0944-7113(11)80030-9, PMID 23195885.
Cao C, Liu Y, Ding Q, Deng Y, Wu Q, Xu Y et al. Effect of oleanolic acid on 5α-reductase activity, DPCs proliferation, and gene expression correlated with androgenetic alopecia in vitro. Acta Med Mediterr. 2019;35:2159-65. doi: 10.19193/0393-6384_2019_4_338.
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Copyright (c) 2023 Arpan Chakraborty, Manas Chakraborty, Goutam Mukhopadhyay, Arka Bhattacharjee, Baishakhi Mondal, Alpana Majumder
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