Caffeine in Tablet Dosage Form: Analytical Method Development and Validation by Using U.V. Spectroscopy
Pharmaceutical Science-Pharmaceutics
DOI:
https://doi.org/10.22376/ijlpr.2023.13.SP1.P107-P115Keywords:
U.V. Spectroscopy, Caffeine, Method Development, Method Validation, Caffeine TabletAbstract
Abstract: Caffeine (1,3,7-trimethylpurine 2,6 dione) is a CNS stimulant. Caffeine is used as a drug having drug-addicted properties having a U.V. cutoff in the range of 272 nm to 297 nm in the U.V. spectrum. In this paper, we took a tablet with Caffeine as a single dose in solid dosage form with excipients that do not have a sharp peak in the U.V. spectrum range from 200 nm to 400 nm. The solvent used as a vehicle is a simple polar solvent with a U.V. cutoff at around 189nm to 191 nm. The U.V. spectrophotometer that we used is of Shimdzu having a double beam, which has two provisions to keep reference and sample at a time to complete sample analysis. Firstly we make a calibration curve of Caffeine with a polar solvent. For the research, development, and production of medicines, analytical technique related to creation and validation are essential. Therefore, in this study, a readily reproducible, straightforward, and quick technique of U.V. spectrophotometric quantitative measurement of Caffeine in the form of tablets is adopted. According to ICH criteria, the parameters to be examined include linearity, specificity, accuracy, precision, and robustness. Purified/deionized water was used as the diluent for the drug quantification at the wavelength of 248.5 nm. Beer-law Lambert's applies to Caffeine at concentrations between 10 and 50 g/ml. Recovery was used to test the method's accuracy, and the results ranged from 99.46 to 100.67%. This approach was used for routine analysis of Caffeine in tablet dosage form.
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Copyright (c) 2022 Dr. Mukesh Kumar Kumawat, Sumit Tewatia, Gufran Ajmal, Pooja Mittal
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