Evaluation of Anti-Inflammatory and Immunomodulatory Activities of Ayapoonaga Chenduram, Seenthil Choornam and their Combination in Wistar Rats
Pharmaceutical Science-Pharmaceutical sciences
DOI:
https://doi.org/10.22376/ijlpr.2023.13.1.P47-55Keywords:
AyapoonagaChendooram, SeenthilChoornam, Anti-Inflammatory Activity, Immunomodulatory Activity, and Hypersensitivity Reaction.Abstract
Ayapoonaga chendooram (APNC) and Seenthilchoornam (SC) are two polyherbal Siddha formulations that traditionally have been used as a folklore medicine to treat rheumatism, orchitis, bronchitis, asthma, tuberculosis (TB), and cough. In the present study, an attempt has been made to demonstrate the antiinflammatory and immunomodulatory activities of individual APNC and SC and also in their combination (APNC+SC). The acute anti-inflammatory effect of APNC, SC and their combination was evaluated in carrageenan-induced hind paw edema in wistar rats. Diclofenac sodium was used as reference drug for comparison. The immunomodulatory activity was evaluated in rats by studying the hemagglutination antibody (HA) titer, delayed type hypersensitivity (DTH) reactions and the Ig E levels. The cyclophosphamide is used as an immunosuppressant.Diclofenac Sodium (50mg/kg) significantly inhibited the carrageenan-induced hind paw edema, indicating its strong anti-inflammatory activity. APNC (65 mg/kg b.w.p.o) at 2nd hour and APNC+SC (13+200 mg/kg b.w.) at 3rd hour had shown moderate inhibition of paw edema, indicating their mild-moderate anti-inflammatory activity but statistically, no significant difference was observed (p>0.05). Oral administration of APNC, SC, and APNC + SC for 21 days increases the left paw volume at 1st hour and 2nd hour resulting in hypersensitivity reaction in rats. The agglutination (dilution factor 1:200) was visually observed in APNC 130mg/kg b.w. (moderate agglutination) and in APNC + SC (high agglutination) results in humoral immunity. Mild increased levels of Ig E were observed in APNC (30 mg/kg b.w.), SC (200 mg/kg b.w.) and in APNC + SC (65+1000 mg/kg b.w.). Under the conditions of the present study, single oral administration of APNC, SC and their combination has shown mild anti-inflammatory activity against carrageenan induced paw edema in experimental rats. The immunity has been stimulated by APNC (13mg/kg b.w.) and SC (2000mg/kg b.w.).
References
Madhavan R, Sathish R, Murugesan M. Standardization of Sanguparpam a herbo marine siddha drug. IntJCurrResChemPharmSci. 2016;3(6):77-84. [SOI: http://s-o-i.org/1.15/ijcrcps-2016-3-6-13].
Ravishankar B, Shukla VJ. Indian systems of medicine: a brief profile. Afr J Tradit Complement AlternMed.2007;4(3):319-37. doi: 10.4314/ajtcam.v4i3.31226, PMID 20161896.
Devi BR, Reddy GD, Kumar GVN, Sathiyarajeswaran P, Elankani P. Herbomineral formulation’s safety and efficacy employed in siddha system of medicine: a review. Int Res J Pharm. 2019;10(1):16-24. doi: 10.7897/2230-8407.10014.
Anupama2017. SeenthilchoornamUsage, Benefits and Side Effects. Bimbima, Dailylife experience of Health, Ayurveda, Medicines, complementary therapies. [Available in online at https://www.bimbima.com/medicines/seenthil-choornam/3953/].
Saha S, Ghosh S. Tinosporacordifolia: One plant, many roles. AncSci Life. 2012;31(4):151-9. doi:10.4103/0257-7941.107344, PMID 23661861.
ReynoldsJW, ReynoldsWM. Earthworms in medicine. Am J Nurs.1972;72(7):1273. doi:10.1097/00000446-197207000-00039, PMID 4555239.
Afreen DS, Shaikh DA. Therapeutic uses of earthworm – areview. IntJ Adv Ayurveda Yoga Unani Siddha Homeopath. 2020;9(1):571-80. doi:10.23953/cloud.ijaayush.469.
Ushakanthan S. Safety and pharmacologicalprofile of SeenthilChooranam [Doctoratethesis], submitted to The Tamilnadu Dr. MGR medical university.Chennai; 2013-2016.
Ramachandran SP.AgasthiarVaidhyaKaviyam.1sted. Vol. 1500.Chennai: Thamarai Publication; 1992,P.5.
Therapeutic index-siddha2010.1sted P 31-2. Published by SKM centre for Ayush Research and Education.
Rahimi HR, Arastoo M, Ostad SN. A comprehensivereview of Punicagranatum (pomegranate) properties in toxicological, pharmacological, cellular and molecularbiologyresearches. Iran J Pharm Res.2012;11(2):385-400. PMID 24250463, PMCID PMC3832175.
FatehMV, AhmedS,Ali M, Bandyopadhyay S.A review on the medicinalimportance of pomegranate. Rajiv Gandhi UnivHealth SciJ PharmSci. 2013;3:23-5.
Amri O, Zekhnini A, Bouhaimi A, Tahrouch S, Hatimi A.Anti-inflammatory activity of methanolic extract from PistaciaatlanticaDesf. leaves. PharmacognJ.2017;10(1):71-6. doi: 10.5530/pj.2018.1.14.
MurunikkaraV, RasoolMK. Trikatu, an herbal compound as immunomodulatory and anti-inflammatory agent in the treatment of rheumatoid arthritis – an experimental study. CellImmunol.2014;287(1):62-8. doi: 10.1016/j.cellimm.2013.12.002, PMID 24394943.
AmdekarS, RoyP, SinghV, KumarA, SinghR, SharmaP. Carrageenan induced oedema in hind paw of the rat as an assay for anti-inflammatory drug. IntJ Inflam. 2012;2012:752015. doi:10.1155/2012/752015, PMID 22518342.
AlseverJB, AinslieR, State NY.Alsever’ssolution. JMed. 1941;126.
Santhammal Y,Arthika S,SharlyElgal N, Ganesan R, Satyarajeswaran P, Gaidhani SNet al.Evaluation of Immunomodulatory Activity of SayaChurnam (A Poly Herbal Formulation) on Albino rats. J Adv Pharm HealthcRes. 2011;1(3).
Ansotegui IJ, Melioli G, Canonica GW, Caraballo L, Villa E, Ebisawa M, et al. IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper. World Allergy Organ J.2020;13(2):100080. doi:10.1016/j.waojou.2019.100080, PMID 32128023.
DjafouaYM, MouokeuRS, TumeC, KamtchuengMO, KuiateJ-R. Immunomodulatory activity of methanol leafextracts of Cameroonian medicinal plants. J Complement IntegrMed.2015;12(4):267-75. doi:10.1515/jcim-2014-0023, PMID 26247508.
TagH, NamsaND, Das AK, Kalita P, Mandal SC.Evaluation of anti-inflammatory potential of Chloranthus erectus (Buch.-Ham.) Verd. leaf extract in rats. J Ethnopharmacol.2009;126(2):371-4. doi: 10.1016/j.jep.2009.08.015, PMID 19703538.
Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, et al.Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl AcadSci U S A.1994;91(25):12013-7. doi: 10.1073/pnas.91.25.12013, PMID 7991575.
Bazzoni F, Beutler B.The tumor necrosis factor ligand and receptor families.NEnglJ Med.1996;334(26):1717-25. doi: 10.1056/NEJM199606273342607, PMID 8637518.
Huang CM, Tsai FJ, Wu JY, Wu MC.Interleukin-1beta and interleukin-1 receptor antagonist gene polymorphisms in rheumatoid arthritis.ScandJRheumatol. 2001;30(4):225-8. doi: 10.1080/030097401316909576, PMID 11578018.
Miller LE. In: Ludke HR, Peacock JE, Tomar RH, editors.Manual of laboratory immunology. Vol. 2.London: Lea &Febiger;1991. p. 1-18.
Askenase PW, Van Loverent H.Delayed-type hypersensitivity: activation of mast cells by antigen-specific T-cell factors initiates the cascade of cellular interactions.Immunol Today.1983;4(9):259-64. doi: 10.1016/0167-5699(83)90046-4, PMID 25290305.
NfambiJ, BbosaGS, SembajweLF, GakungaJ, KasoloJN. Immunomodulatory activity of methanolic leaf extract of Moringaoleifera in Wistar albino rats. J Basic ClinPhysiolPharmacol.2015;26(6):603-11. doi: 10.1515/jbcpp-2014-0104, PMID 26103628.
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Copyright (c) 2022 G. Dayanand Reddy, M. Kannan, P. Sathiya Rajeswaran, R. Ganesan, B. Rama Devi, K. Dhanaraj, D. Pitchaiah
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