Identification of Scardovia Wiggsiae in Children Between Severe Early Childhood Caries and Caries-Free Children – A Polymerised Chain Reaction -Based Study
Life Sciences-Medicine
DOI:
https://doi.org/10.22376/ijpbs/lpr.2022.12.6.SP25.L116-122Keywords:
Early Childhood Caries, Scardovia Wiggsiae, Polymerase Chain ReactionAbstract
Dental caries is the most common microbial disease of oral cavity, affecting 60 – 90% of children worldwide. Early Childhood Caries (ECC) is a major health problem in developing countries. These lesions are destructive and can affect the development of permanent successors. Although Streptococcus mutans is the primary cause of ECC, some studies support the view that caries can develop in the absence of mutans streptococci. Scardovia wiggsiae, a gram-positive anaerobe was recently found to be associated with Early Childhood Caries in the presence and absence of Streptococcus mutans. The aims were to investigate the cariogenic potential of S. wiggsiae, in ECC. The study was intended to compare the presence of S. wiggsiae, in the saliva samples of caries-free children and children with severe ECC, thereby determine this new acidogenic species noxiousness as a pathogen in Early Childhood Caries.Saliva samples were collected from 40 children, age ranging between three to six years. The sample thus collected at room temperature was then subjected to DNA extraction and Polymerase Chain Reaction (PCR) analysis to evaluate the presence of S. wiggsiae. Numerical parameters were tested for significance using Fisher’s Exact Test and the statistical analysis using Epi Info software.The tested samples revealed almost equal proportion of Scardovia wiggsiae in caries-free group and severe ECC group. S. wiggsiae was isolated and detected in both the study and the control groups. Statistical significance was not pronounced claiming the falsehood of the association between Early childhood caries and Scardovia wiggsiae.
References
Hu S, Sim YF, Toh JY, Saw SM, Godfrey KM, Chong YS et al. Infant dietary patterns and early childhood caries in a multi-ethnic Asian cohort. Sci Rep. 2019 Jan 29;9(1):852. doi: 10.1038/s41598-018-37183-5, PMID 30696871.
Nair S, Kumar VS, Krishnan R, Rajan P. A comparative evaluation of bifidobacteria levels in early childhood caries and severe early childhood caries. J Pharm Bioallied Sci. 2017 Nov;9(Suppl 1);Suppl 1:S82-4. doi: 10.4103/jpbs.JPBS_75_17, PMID 29284941.
Kashyap P. Scardovia wiggsiae - A new etiological agent for early childhood caries. Int J Appl Dent Sci. 2020;6(4):177-80. doi: 10.22271/oral.2020.v6.i4c.1064.
Anil S, Anand PS. Early childhood caries: prevalence, risk factors, and prevention. Front Pediatr. 2017 Jul 18;5:157. doi: 10.3389/fped.2017.00157, PMID 28770188.
Hemadi AS, Huang R, Zhou Y, Zou J. Salivary proteins and microbiota as biomarkers for early childhood caries risk assessment. Int J Oral Sci. 2017 Nov;9(11):e1. doi: 10.1038/ijos.2017.35, PMID 29125139.
Tinanoff N, Baez RJ, Diaz Guillory C, Donly KJ, Feldens CA, McGrath C et al. Early childhood caries epidemiology, aetiology, risk assessment, societal burden, management, education, and policy: global perspective. Int J Paediatr Dent. 2019 May;29(3):238-48. doi: 10.1111/ipd.12484, PMID 31099128.
Cabral MBBS, Mota ELA, Cangussu MCT, Vianna MIP, Floriano FR. Risk factors for caries-free time: longitudinal study in early childhood. Rev Saude Publica. 2017 Dec 4;51:118. doi: 10.11606/S1518-8787.2017051006558, PMID 29211203.
Abbate GM, Borghi D, Passi A, Levrini L. Correlation between un-stimulated salivary flow, pH and streptococcus mutans, analysed. Eur J Pediatr Dent. 2014 Jan;15:1.
Arefi AH, Shamsaddin H, Balvardi M, Poureslami H, Danesh M, Sayadizadeh M. Evaluation of parents’ views about etiologic factors of severe early childhood caries: A qualitative study. J Dent Res Dent Clin Dent Prospects. 2019;13(1):43-50. doi: 10.15171/joddd.2019.007, PMID 31217918.
Chandna P, Srivastava N, Sharma A, Sharma V, Gupta N, Adlakha VK. Isolation of Scardovia wiggsiae using real-time polymerase chain reaction from the saliva of children with early childhood caries. J Indian Soc Pedod Prev Dent. 2018 Jul 1;36(3):290-5. doi: 10.4103/JISPPD.JISPPD_225_17, PMID 30246752.
Kameda M, Abiko Y, Washio J, Tanner ACR, Kressirer CA, Mizoguchi I et al. Sugar metabolism of Scardovia wiggsiae, a novel caries-associated bacterium. Front Microbiol. 2020 Mar 25;11:479. doi: 10.3389/fmicb.2020.00479, PMID 32269556.
Vacharaksa A, Suvansopee P, Opaswanich N, Sukarawan W. PCR detection of Scardovia wiggsiae in combination with Streptococcus mutans for early childhood caries‐risk prediction. Eur J Oral Sci. 2015 Oct;123(5):312-8. doi: 10.1111/eos.12208, PMID 29917306.
Tanner AC, Mathney JM, Kent RL, Chalmers NI, Hughes CV, Loo CY et al. Cultivable anaerobic microbiota of severe early childhood caries. J Clin Microbiol. 2011 Apr;49(4):1464-74. doi: 10.1128/JCM.02427-10, PMID 21289150.
Hurley E, Barrett MPJ, Kinirons M, Whelton H, Ryan CA, Stanton C et al. Comparison of the salivary and dentinal microbiome of children with severe-early childhood caries to the salivary microbiome of caries-free children. BMC Oral Health. 2019 Dec;19(1):13. doi: 10.1186/s12903-018-0693-1, PMID 30642327.
Downes J, Mantzourani M, Beighton D, Hooper S, Wilson MJ, Nicholson A et al. Scardovia wiggsiae sp. nov., isolated from the human oral cavity and clinical material, and emended descriptions of the genus Scardovia and Scardovia inopinata. Int J Syst Evol Microbiol. 2011 Jan 1;61(1):25-9. doi: 10.1099/ijs.0.019752-0, PMID 20139283.
Wang Y, Zhang J, Chen X, Jiang W, Wang S, Xu L et al. Profiling of oral microbiota in early childhood caries using single-molecule real-time sequencing. Front Microbiol. 2017 Nov 15;8:2244. doi: 10.3389/fmicb.2017.02244, PMID 29187843.
Row L, Repp MR, Kingsley K. Screening of a pediatric and adult clinic population for caries pathogen Scardovia wiggsiae. J Clin Pediatr Dent. 2016;40(6):438-44. doi: 10.17796/1053-4628-40.6.438, PMID 27805882.
Carr G, Alexander A, Nguyen L, Kingsley K. Oral site-specific sampling reveals differential location for Scardovia wiggsiae. Microbiol Res J Int. 2020 Feb 19:47-55. doi: 10.9734/mrji/2020/v30i130189.
McDaniel J, McDaniel S, Tam A, Kingsley K, Howard KM. Screening a saliva repository for Scardovia wiggsiae and Streptococcus mutans: A pilot study. Adv Microbiol. 2017 Aug 29;5(1):1-8. doi: 10.9734/JAMB/2017/36111.
Vieira AR, Hiller NL, Powell E, Kim LH, Spirk T, Modesto A et al. Profiling microorganisms in whole saliva of children with and without dental caries. Clin Exp Dent Res. 2019 Aug;5(4):438-46. doi: 10.1002/cre2.206, PMID 31452955.
Prabhu Matondkar SP, Yavagal C, Kugaji M, Bhat KG. Quantitative assessment of Scardovia wiggsiae from dental plaque samples of children suffering from severe early childhood caries and caries free children. Anaerobe. 2020 Apr 1;62:102110. doi: 10.1016/j.anaerobe.2019.102110, PMID 31629044.
Zhou J, Jiang N, Wang S, Hu X, Jiao K, He X et al. Exploration of human salivary microbiomes—insights into the novel characteristics of microbial community structure in caries and caries-free subjects. PLOS ONE. 2016 Jan 19;11(1):e0147039. doi: 10.1371/journal.pone.0147039, PMID 26784334.
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