Study On Phytochemical and Antioxidant Properties of Padina gymnospora and Ulva lactuca
Life Sciences-Marine Biology
DOI:
https://doi.org/10.22376/ijpbs/lpr.2022.12.6.L155-160Keywords:
Padina gymnospora, Ulva lactuca, Phytochemical, Antioxidants and, MethanolAbstract
Seaweed constitutes one of the commercially important living renewable resources in the marine ecosystem.Seaweeds have antimicrobial, antioxidant, and anti-inflammatory properties. The aim of the study is to estimate the phytochemicals and antioxidant activity of Padina gymnospora and Ulva lactuca. The antioxidant properties and phytochemical analysis of Padina gymnospora and Ulva lactuca collected from the Gulf of Mannar during the low tide period were analyzed.The study's objective is to analyze the phytochemical and antioxidant activities using methanol as solvent. Antioxidant activities were determined using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay treated with methanol and Phytochemical analysis carried out by Folin-Ciocalteu reagent. Antioxidants and phytochemicals in seaweeds provide healthpromoting effects and protect against non-communicable diseases such as Cancer, arthritis, diabetes, obesity, etc. The results reveal that antioxidant activities were higher in Padina gymnospora in methanol solvent than in Ulva lactuca. The percentage of inhibition of Padina gymnospora and Ulva lactuca was 61.5 and 64.5 respectively. IC50 value of Padina gymnospora and Ulva lactuca was 60.4 and 60.6 respectively.The preliminary phytochemical analysis of Padina gymnospora and Ulva lactuca was found to contain Alkaloids, Steroids, Terpenoids, Glycosides. To know the efficiency of green seaweed Ulva lactuca with brown seaweed Padina gymnospora the study was carried out. The highest flavonoid content was recorded in brown seaweed Padina gymnospora (25.5±0.02mg REg-1) compared toUlva lactuca (19.32±0.5mg REg-1). The total phenolic content was highest in Padina gymnospora (3.7±0.06mg GAEg-1) whereas Ulva lactuca contained (2.81±0.3mg GAEg-1). Therefore, the present work recommends consuming seaweeds in our regular diets to have a healthy lifestyle. From the current study of phytochemical and antioxidant rich seaweeds can be incorporated in the formulation of drugs used for treating various non- communicable diseases prevailing in the present era. Keywords: , , ,
References
AlaganVT, ValsalaRN, RajeshKD. Bioactive chemical constituent analysis, in vitro antioxidant and antimicrobial activity of whole plant methanol extracts of Ulva lactuca Linn, BJPR. 2017;15(1):1-14.
Balalakshmi C, Rajkumar G, Balamurugan V, Krishnan V, Arumugam A, Sabitha Vet al.Preliminary phytochemical Screenings of Marine alga Ulva fasciata and its growth performance, biochemical composition on Indian major carp Cirrhinus mrigala fingerlings JOAASR-Vol-1-10; 2017.
Maheswari MU, Reena A, Sivaraj C. GC-MS analysis, antioxidant and antibacterial activity of the brown algae, Padina tetrastromatica. Int J Pharm Sci Res. 2017;8(9):4014-20.doi: 10.13040.
RajA, JeganS, ChandrasekaranM, VenkatesaluV. Phytochemical analysis and comparison of antibacterial activity of various solvent extracts of Caulerpa racemosa on multidrug resistant bacterial strains Sciencia.ActaXaverianaIntSciJ. 2017;8 No. 2:43-57.
Abirami RG, Kowsalya S. Phytochemical screening, microbial load and antimicrobial activity of underexploited seaweeds International.ResJ Microbiol. 2012;3(10):328-32.
FarasatM, Khavari-NejadR-A, NabaviSMBN, Namjooyan F. Antioxidant activity, total phenolics and flavonoid contents of some Edible Green Seaweeds from Northern Coasts of the Persian Gulf. IranJ PharmRes. 2014;13(1):163-70. PMID 24734068.
Matanjun P, Mohamed S, Mustapha NM, Muhammad K, Ming CH. Antioxidant activities and phenolics content of eight species of seaweeds from north Borneo. J ApplPhycol. 2008;20(4):367-73. doi: 10.1007/s10811-007-9264-6.
Carocho M, Ferreira ICFR. A review on antioxidants, pro-oxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food ChemToxicol. 2013; 51:15-25. doi: 10.1016/j.fct.2012.09.021, PMID 23017782.
Kang MC, Kang N, Kim SY, Lima IS, Ko SC, Kim YTet al. Popular edible seaweed, Gelidium amansii prevents against diet induced obesity. Food ChemToxicol. 2016; 90:181-7. doi: 10.1016/j.fct.2016.02.014, PMID 26911551.
Tegeli V, Karpe P, Katve V. Importance of free radical and antioxidant on human health. IntJ PharmChemBiolSci. 2014;4(4):1038-50.
Nithya P, Loganathan S, Maruthupandian A, Viji M, Murugan P. Phytochemical evaluation and bioactive potential of brown seaweeds. Int. J Adv Inter dis Res. 2017;4(4):4-9.
CyrilR, LakshmananR, ThiyagarajanA. In vitro bioactivity and phytochemical analysis of two marine macro-algae. JCLM.2017;5(10):427-32. doi: 10.12980/jclm.5.2017J7-124.
Raaman N. Phytochemical techniques. New Delhi: New India Publishing Agency; 2006. p. 306.
Trease GE, Evans WC. Textbook of pharmacognosy. 12thed.London: Balliese Tindall and Company Publisher;1983. p. 343-83.
Siddhuraju P, Becker K. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extracts. Food Chem. 2007;101(1):10-9. doi: 10.1016/j.foodchem.2006.01.004.
Brighente IMC, Dias M, Verdi LG, Pizzolatti MG. Antioxidant activity and total phenolic content of some Brazilian species. PharmBiol. 2007;45(2):156-61. doi: 10.1080/13880200601113131.
Zhang WW, Duan XJ, Huang HL, Zhang Y, Wang BG. Evaluation of 28 marine algae from the Qingdao coast for antioxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and subfractions derived from Symphyocladia latiuscula (Rhodomelaceae). J ApplPhycol. 2007;19(2):97-108. doi: 10.1007/s10811-006-9115-x.
ThinakaranT, BalamuruganM,SivakumarK. Screening of phycochemicals qualitatively and quantitatively certain seaweeds from Gulf of Mannar Biosphere Reserve. IRJP, 3. 2012;7.
Saranya C, Parthiban C, Anantharaman P. Evaluation of antibacterial and antioxidant activities of seaweeds from Pondicherry coast. AdvApplSciRes. 2014; 5:82-90.
Leelavathi MS, Prasad M. Comparative analysis of phytochemical compounds of marine algae isolated from Guld of Mannar. World J PharmSci. 2015; 4:640-54.
Thamizharasan S. Phytochemical compounds of Padina gymnospora and its mic effects. The PharmInnovJ. 2018;7(11):512-5.
Boisvert C, Beaulieu L, Bonnet C, PelletierÉ. Assessment of the antioxidant and antibacterial activities of three species of edible seaweeds. J Food Biochem. 2015;39(4):377-87. doi: 10.1111/jfbc.12146.
Jeeva S,Marimuthu J, Domettila C, Anantham B, Mahesh M.Preliminary phytochemical studies on some selected seaweeds from Gulf of Mannar, India. Asian PacJ TropBiomed. 2012;2(1): S30-3. doi: 10.1016/S2221-1691(12)60125-7.
Iloki-AssangaSB, Lewis-LujánLM, Lara-EspinozaCL, Gil-SalidoAA, Fernandez-Angulo D, Rubio-PinoJL et al. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum.BMCRes Notes. 2015; 8:396. doi: 10.1186/s13104-015-1388-1, PMID 26323940.
Trigui M, Gasmi L, Zouari I, Tounsi S. Seasonal variation in phenolic composition, antibacterial and antioxidant activities of Ulva rigida(Chlorophyta) and assessment of anti-acetylcholinesterase potential. JApplPhycol. 2013;25(1):319-28. doi: 10.1007/s10811-012-9866-5.
Valliappan K, Sun W, Li Z. Marine action bacteria associated with marine organisms and their potentials in producing pharmaceutical natural products. ApplMicrobiolBiotechnol. 2014;98(17):7365-77. doi: 10.1007/s00253-014-5954-6, PMID 25064352.
ThanigaivelS, Vidhya Hindu SV, VijayakumarS, MukherjeeA, ChandrasekaranN, ThomasJ. Differential solvent extraction of two seaweeds and their efficacy in controlling Aeromonas salmonicida infection in Oreochromis mossambicus: A novel therapeutic approach. Aquaculture. 2015; 443:56-64. doi: 10.1016/j.aquaculture.2015.03.010.
Hebsibah Elsie B, Sudha PN, Dhanarajan S. In vitrostudies on antimicrobial analysis of marine algae (seaweeds) Sargassum wightii and Gracillaria edilus using three different solvents extracts. The Ecoscan. 2010; 4:343-5.
SznarkowskaA, KosteckaA, MellerK, BielawskiKP. Inhibition of cancer antioxidant defense by natural compounds. Onco target. 2017;8(9):15996-6016. doi: 10.18632/oncotarget.13723, PMID 27911871.
FoonTS, Lian Ai Ai , KuppusamyP, YusoffMM, GovindanN. Studies on in-vitro antioxidant activity of marine edible seaweeds from the east coastal region of Peninsular Malaysia using different extraction methods.J Coast Life Med. 2013;1(3):193-8.
RamahS, EtwarysingL, AucklooN, GopeechundA, BhagoolR, BahorunT. Prophylactic antioxidants and phenolics of seagrass and seaweed species: A seasonal variation study in a Southern Indian Ocean Island, Mauritius.Internet J Med Update. 2014;9(1):27-37.
Meenakshi S, Manicka GD, Tamilmozhi S, Arumugam M, Balasubramanian T. Total flavanoid and in vitro antioxidant activity of two seaweeds of Rameshwaram coast. Glob J Pharmacol. 2009; 3:59-62.
Manivannan K, Thirumaran G, Devi GK, Hemalatha A, Anantharaman P. Biochemical composition of seaweeds from Mandapam coastal regions along Southeast Coast of India. Am Eurasian J Botany. 2008;1:32-7.
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