Pharmacognostic and Phytochemical Features of Andrographis echioides Whole Plant Powder
Life Sciences-Microbiology
DOI:
https://doi.org/10.22376/ijpbs/lpr.2022.12.5.L196-205Keywords:
Andrographis echioides, Pharmacognosy, Phytochemistry, Histochemistry, Whole plant.Abstract
Andrographis echioides is one of the stress tolerant plant that belongs to the family Acanthaceae commonly called as Kopuramthangi in Tamil, commonly found in central region of Tamilnadu, India. Local peoples used this plant for fever, to relieve griping, irregular stools and loss of appetite. Indian siddha and ayurvedic pharmacopeia provide standardization criteria for herbal drugs. The aim of the study is to provide quality standards for the powder drug from the whole plant of Andrographis echioides. In order to provide herbal standards for this plant, the present study was undertaken with the objectives to assess pharmacognostic, physiochemical and phytochemical markers of the drug powder of Andrographis echioides whole plant. Andrographis echioides whole plant was collected, authenticated and processed for all pharmacognostic activity using standard textual methods. Powder analysis showed the presence of calcium oxalate crystals, trichomes, fibres, vessel elements and parenchyma cells. The powder is yellowish green in colour with 3.6% ash value with higher water extractive (19.4%). Powder contains a smaller number of microbial flora and no pathogens detected. Multiple fluorochromes were detected in fluorescence test. The pharmacognostic results were within the limits of ayurvedic pharmacopeia of India. Whole plant powder showed the presence of terpenoids, flavonoids, Saponins, phenolic compounds and carbohydrates which could be responsible for all biological activities of the plant. Histochemical analysis also confirmed the availability of phytoconstituents like flavonoids and tannins. The observations made through this study would be of immense value in the pharmacological evaluation and standardization of crude powder of the plant materials.
References
Akbar S, Hanif U, Ali J, Ishtiaq S. Pharmacognostic studies of stem, roots and leaves of Malva parviflora L. Asian Pac J Trop Biomed. 2014;4(5):410-5. doi: 10.12980/APJTB.4.2014C1107, PMID 25182728.
Amponsah IK, Mensah AY, Otoo A, Mensah MLK, Jonathan J. Pharmacognostic standardisation of Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae). Asian Pac J Trop Biomed. 2014;4(12):941-6. doi: 10.12980/APJTB.4.2014APJTB-2014-0416.
Brady L, Robbers J, Tyler V. Pharmacognosy. 9th ed. Philadelphia: Lea & Febiger; 1988.
Mahady GB. Global harmonization of herbal health claims. J Nutr. 2001;131(3s):1120S-3S. doi: 10.1093/jn/131.3.1120S, PMID 11238830.
Von Bargen KW, Niehaus EM, Bergander K, Brun R, Tudzynski B, Humpf HU. Structure elucidation and antimalarial activity of apicidin F: an apicidin-like compound produced by Fusarium fujikuroi. JNat Prod. J Nat Prod. 2013;76(11):2136-40. doi: 10.1021/np4006053, PMID 24195442.
Orhan IE. Pharmacognosy: science of natural products in drug discovery. BioImpacts. 2014;4(3):109-10. doi: 10.15171/bi.2014.001, PMID 25337461.
Yoganarasimman SN. Medicinal plants, Regional research institute (AY), Bangalore. 2000; 2:343, 452.
Asher GN, Corbett AH, Hawke RL. Common herbal dietary supplement-drug interactions. Am Fam Physician. 2017;96(2):101-7. PMID 28762712.
Gamble JS. Flora of the presidency of Madras. Botanical Survey of India. Calcutta: 1956; 1051.
Ruba A, Mohan VR. In vitro antioxidant potential of whole plant of Andrographis echioides (L.) Nees (Acanthaceae). Int J Pharmaand Pharmaceu Sci. 2016;8(6).
Muralidharan K, Kumaravelu P, David DC. Cytotoxic effect of ethanolic extracts of Andrographis echioides in human colon cancer cell Lines via Apoptosis. Res J Pharm Technol. 2020;13(2):871-6. doi: 10.5958/0974-360X.2020.00165.1.
Pandi Kumar P, Ayyanar M. Lgnacimuthu S. Medicinal plants used by Malasar tribes of Coimbatore district, Tamil Nadu. Indian J Trad Knowl. 2007; 6:579-82.
Nadkarni AK, Nadkarni KM. Indian materia medica. Vol. 1. Bombay: Popular Press Prakashan; 1976.
Qadrie ZL, Jacob B, Anandan R, Rajkapoor B, Rahamathulla M. Anti-bacterial activity of ethanolic extract of Indoneesiella echioides (L) nees. evaluated by the filter paper disc method. Pak J Pharm Sci. 2009;22(2):123-5. PMID 19339219.
De-Yang S, Juang S-H, Kuo P-C, Huang G-J, Chan Y-Y, Amooru G. DamuandTian-Shung Wu. 2013. Chemical Constituents from Andrographis echioides and Their Anti-Inflammatory Activity. Inte J Mol Sci; 14:496-514.
Ramasubramania Raja R, Jeevanreddy K. Pharmacognostical phytochemical and antiulcer activity of Andrographis echioides (Acanthaceae). J Pharmacogn Phytochem. 2014;3(3):39-49.
Mathivanan D, Suseem SR. Phytochemical and pharmacological review of Andrographis echioides. J Chemand Pharm Res. 2015;7(7):1167-71.
Anonymous. Indian pharmacopoeia. Gov India Minist Health Fam Welf Controller Publ Civ Lines Delhi– 110054. 1996; I & II.
Siddiqui A, Hakim MA. Format for the pharmacopoeial analytical standards of compound formulation, workshop on standardization of Una ni drugs, (appendix), 24-25 January. New Delhi: central council for research in Una ni Medicine (CCRUM); 1995.
Wallis TE. Textbook of pharmacognosy. 5th ed, CBS Publishers and Distributors. New Delhi, India; 2005.
Kabuki T. Characterization of novel antimicrobial compounds from mango (Mangifera indica L.) kernel seeds. Food Chem. 2000;71(1):61-6. doi: 10.1016/S0308-8146(00)00126-6.
Brindha P, Sasikala P, Purushothaman KK. Pharmacognostic studies on merugan kizhangu, Bull in Med Ethics and Bot Res. 1981;3:84-96.
Chase CR, Pratt RS. Fluorescence of Powdered Vegetable drugs with particular reference to Development of a System of Identification. J Am Pharmacol. Association. 1949; 38:32.
Kokate CK. Practical pharmacognosy. 1st ed. New Delhi: Vallabh Prakashan; 1986. p. 111.
Sofowara A. Medicinal plants and Traditional medicine in Africa. Ibadan, Nigeria: Spectrum Books Ltd; 1993. p. 289.
Trease GE, Evans WC. Pharmacognsy. 11th ed; 1989 Brailliar Tiridel Can. Macmillian publishers.
Harborne JB. Phytochemical Methods; A guide to modern techniques of plant Analysis. 2nd ed. London, New York; 1973. p. 49-188.
Harborne JB. Phytochemical Methods.A Guide to Modern Technique of Plant analysis. London: Chapman & Hall; 1984. p. 78-210.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4(7):685-8. doi: 10.5897/AJB2005.000-3127.
Van-Burden TP, Robinson WC. Formation of complexes between protein and tannin acid. J Agric Food Chem. 1981; 1:77-82.
Obdoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some Homostatic plants in Edo and Delta States of Nigeria. Glob J Pure Appl Sci. 2001; 8:203-8.
Boham BA, Kocipai-Abyazan R. Flavonoids and condensed tannins from leaves of Vaccinium vaticulatum and V. calycinium. Pac Sci. 1974; 48:458-63.
John Peter Paul J. Histochemistry and fluorescence analysis of Turbinaria ornata (Turner) J. Ag. –An important brown seaweed (Phaeophyceae). Ind JPlant sci. 2014;3(1):40-4.
Gersbach P. A new histochemical method for localization of the site of monoterpene phenol accumulation in plant secretory structures. Annals of Botany. 2001;88(4):521-5. doi: 10.1006/anbo.2001.1480.
Anandanayaki S, Uma C. Pharmacognostical and phytochemical studies on Andrographis echioides (L.) Nees. Int J Adv Pharm Biol Chem. 2014;3(3):640-5.
Lala PK. Practical pharmacognosy, 1. New Delhi: Vallabh Prakashan; 1981. p. 86-95.
Keher C. Herbal medicine. Curr Sci. 1946; 15:48.
Ajila CM, Prasada Rao UJ. Protection against hydrogen peroxide induced oxidative damage in rat erythrocytes by Mangifera indica L. peel extract. Food Chem Toxicol. 2008;46(1):303-9. doi: 10.1016/j.fct.2007.08.024, PMID 17919803.
Handa SS, Sharma A, Chakraborti KK. Natural products and plants as liver protecting drugs. Fitoterapia. 1986; 57:307-45.
Harborne JB. Phytochemical methods, A guide to modern techniques of plant analysis, Champman, hall, 3. New York; 1998.
Abraham A, Mathew L, Samuel S. Pharmacognostic studies of the fruits of Terminalia bellirica (Gaertn.) Roxb. J Pharmacogn Phytochem. 2014;3(2):45-52.
Kumari, Mythili Krishna, Joshi, Gurav, Anant V Bhandarkar, Agarwal, Deepak M and Gururaj GM. A pharmacognostic, phytochemical and pharmacological review of Terminalia bellerica. J Pharmacogn Phytochem. 2017;6(5):368-76.
Shen DY, Juang SH, Kuo PC, Huang GJ, Chan YY, Damu AG et al. Chemical constituents from Andrographis echioides and their anti-inflammatory activity. Int J Mol Sci. 2012;14(1):496-514. doi: 10.3390/ijms14010496. PMID 23271366.
Govindachari TR, Parthasarathy PC, Pai BR, Subramaniam PS. Chemical examination of andrographis echioides—I. Tetrahedron. 1965;21(9):2633-40. doi: 10.1016/S0040-4020(01)93919-5.
Govindachari TR, Parthasarathy PC, Pai BR, Subramaniam PS. Chemical examination of Andrographis echioides—II. Tetrahedron. 1965;21(12):3715-20. doi: 10.1016/S0040-4020(01)96987-X.
Jayaprakasam B, Damu AG, Gunasekar D, Blond A, Bodo B. Dihydroechioidinin, a flavanone from Andrographis echioides. Phytochemistry. 1999;52(5):935-7. doi: 10.1016/S0031-9422(99)00295-2.
Jayaprakasam B, Gunasekar D, Rao KV, Blond A, Bodo B. Androechin, a new chalcone glucoside from Andrographis echioides. J Asian Nat Prod Res. 2001;3(1):43-8. doi: 10.1080/10286020108042837, PMID 11355769.
Priya RR, Bhaduhsha N. Veramuthu Manivannan and Thanthoni Gunasekaran. Preliminary quantitative phytochemical, physicochemical and in-vitro antioxidant activity of Senna alata hydroalcoholic leaf extract. Int J Life Sci Pharm Res. 2022;12(1):95-102.
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