Development of optimized formulation of liposome using 3-factor Box-Behnken Design

Pharmaceutical Science-Pharmacy for better health

Abstract

The  objective  of  our  present  study  is  to  optimize  Chitosan  coated  liposomes  formulation  by Response surface methodology using 3-factor Box-Behnken Design. Different polymers based liposomes used for delivery of stable pH dependent formulation. Chitosan has been used as a pH sensitive polymer coating to target  nanoparticles specifically  to  tumors  which  have  a  slightly  acidic pH. Closed  membrane  system  can accommodate amphiphilic or lipophilic drugs in vesicles. The optimized batch was formulated as a liposome delivery system and evaluation was done. To evaluate the unentrapped drug Shimadzu UV-Spectrometry at 228 nm was used and absorbance was noted. Response surface graph was prepared to predict value and the optimized formulation (Chitosan coated liposomes) can be used for loading of bio-active.The values of percent drug entrapment and average vesicle size were presented and found formulation F3&F5 were optimized for further evaluating on basis of particle size and drug loading.