Biometric Estimation for Understanding the Nature of Vorticella Stalk Contraction- Extension Repeated Consequential Cyclic Processes

Life Sciences-Chemistry for New drug discovery

Abstract

Vorticella stalk is the storehouse  of two types of novel proteins, known with the names of spasmins and batonnets. On the basis of nature of proteins and the arrangements  of amino acid residues of these proteins, the repeated consequential  cyclic processes  of contraction dynamics worked by neutralizing the negatively charged amino acid residues as per the laws obeyed by Heber-Weiss, Nernst and Fenton reactions. H+-integrated physiological performances in combination with pCa (partial pressure of calcium ion concentrations) and DNFB (2,4 – dinitrofluorobenzene/Sanger’s reagent) concentration  gradients at the range of 1mM to 5mM represented velocity inclination in acidic medium which were more actively pronounced  if it was compared with alkaline medium where permeabilized  stalk exaggerated  potential biochemical- shift-perturbation  if it was in respect of non-permeabilized  live specimens in both artificial as well as in natural medium in different experimental trials under controlled  electro-physiological  instrumental  setup conditions.  On the basis of these experimental  designs it was confirmed  that spasmins and batonnets are two different types of novel proteins with multitudes of potential applications in the favour of biomedical engineering devices formulation, then their construction at the nano-scale where H+-integrated pCa dependent electrophysiological nature of recommended proteins were found more ROS (reactive oxygen species) resistant if there was the introduction  of DNFB in fixed concentrations  than in the acto-myosin  as well as tubulin-dynein  systems being exclusively controlled under post-translational  biochemical reactions   catalysed in the light of  software  based  modern  bioinformatics’  tools  and  techniques.  In  live  as  well  as  permeabilized  specimens,  different  types  of biochemical reaction kinetics of amino acid residues were performed at different rates among the sequentially determined  spasmins and batonnets like novel proteins   where   molecular   orientations   and   motive-force   generation   in   measurable   parameters   per   millisecond   confirmed   the electrophysiological  significance of Vorticella stalks’ on the basis of colligative nature of novel proteins of saccular compartments of spasmoneme, the well explained active contractile organelle of the stalk in relation with other resembling proteins found in Protein Data Bank (PDB) as centrins, calmodulins and others significantly pronounce their life saving and medicinal properties. This present statement/study was aimed to know the biochemical  behaviour  of spasmins and batonnets like novel proteins in the light of electrochemical  behavior of the Vorticella stalk under some selective chemical stress conditions, that’s why this research helped us to know the ROS resistance properties of novel protein polymers found in stalk. On the basis of which reference proteins as described in this paper can be used as a diagnostic tools in pharmaceutical industries in the favour of molecular medicines and drugs’ designing.