SAFFRON (CROCUS SATIVUS) AMELIORATES TNBS-INDUCED COLITIS IN RATS VIA DOWNREGULATION OF INFLAMMATORY CYTOKINES TNF-α AND IL- 10, CASPASES-3 GENE EXPRESSION AND OXIDATIVE STRESS IN EXPERIMENTAL RATS

Pharmaceutical Science-Pharmacology

Authors

  • ZAFER SAAD ALSHEHRI College of Applied Medical Sciences , Shaqra University , Aldwadmi , Saudi Arabia
  • WIDAD M. AL-BISHRI Biochemistry Department, King Abdulaziz University, Faculty of Science, Jeddah, Saudi Arabia
  • RASHA H. HUSSEIN Biochemistry Department, King Abdulaziz University, Faculty of Science, Jeddah, Saudi Arabia. Department of Biochemistry and Nutrition, Women College, Ain Shams University, Cairo, Egypt
  • MANAL E. EL-HALWAGY Biochemistry Department, King Abdulaziz University, Faculty of Science, Jeddah, Saudi Arabia

Keywords:

Saffron extract - Colitis- TNBS- Caspase-9 gene expression - Sucrose - Cytokines

Abstract

Ulcerative colitis (UC) is a chronic replacing disorder of the gastrointestinal tract by inflammation and tissue damage. Saffron, a spice derived from the flower of Crocus sativus, is rich in carotenoids . In the current study, we aimed to investigate the potential alleviating effects of Saffron extract against TNBS-induced colitis in rats. saffron extract (80 ml/kg body weight  was administered daily by oral gavages in ulcerative colitic rats. Administration of saffron extract mitigated the colonic levels of TNF-a and IL- 10 cytokines. The attenuation of saffron extract to colon injury was also associated with suppression of oxidative stress via reduction of lipid peroxides and nitric oxide along with boosting the antioxidant defenses through restoration of colon glutathione. In addition, caspases-3 gene expression activity, an apoptotic marker, was inhibited. Furthermore, addition of sucrose on the diet of colitic rats increases the colonic inflammation, without incidence on the antioxidant status of colitic rats, indicating that the increase in oxidative stress is directly related to TNBS rather than sucrose.

Published

2022-07-11

How to Cite

ZAFER SAAD ALSHEHRI, WIDAD M. AL-BISHRI, RASHA H. HUSSEIN, & MANAL E. EL-HALWAGY. (2022). SAFFRON (CROCUS SATIVUS) AMELIORATES TNBS-INDUCED COLITIS IN RATS VIA DOWNREGULATION OF INFLAMMATORY CYTOKINES TNF-α AND IL- 10, CASPASES-3 GENE EXPRESSION AND OXIDATIVE STRESS IN EXPERIMENTAL RATS: Pharmaceutical Science-Pharmacology . International Journal of Life Science and Pharma Research, 7(1), 1–9. Retrieved from https://www.ijlpr.com/index.php/journal/article/view/1088

Issue

Volume 7 Issue 1, January - March 2017

Section

Research Articles
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